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| Registros recuperados: 225 | |
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| Renault, Tristan; Cochennec, Nathalie; Grizel, Henri. |
| The protozoan Bonamia ostreae (Ascetospora) is the etiological agent of bonamiosis, or hemocyte disease of the European fiat oyster, Ostrea edulis (Pichot et al., 1980). Since this infection was first reported from the coast of Brittany (Comps et al., 1980), the parasite has spread rapidly along the French Atlantic coasts (Grizel & Tigé, 1982), and in the European countries including the UK, Holland, lreland and Spain (Bannister & Key, 1982; Van Banning, 1982; Polanco et al., 1984). Under the EEC Council Directive 91/67, this parasitic protozoan is included in list II of Annex A. Serious pathogen agents for molluscs that should be obligatorily declared are placed in this list |
| Tipo: Text |
Palavras-chave: Infection; Protozoan; Oyster; Ostrea edulis; Bonamia ostreae. |
| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/1995/publication-2892.pdf |
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| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf |
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| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf |
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| Harrang, Estelle; Heurtebise, Serge; Faury, Nicole; Robert, Maeva; Arzul, Isabelle; Lapegue, Sylvie. |
| The present study identifies quantitative trait loci (QTLs) in response to an experimental infection with the parasite responsible for bonamiosis, Bonamia ostreae, in two segregating families of the European flat oyster, Ostrea edulis. We first constructed a genetic-linkage map for each studied family and improved the existing genetic-linkage map for the European flat oyster with a set of SNP markers. This latter map now combines the best accuracy and the best estimate of the genome coverage available for an oyster species. Secondly, by comparing the QTLs detected in this study with those previously published for O. edulis in similar experimental conditions, we identified several potential QTLs that were identical between the different families, and also... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Single Nucleotide Polymorphism; Disease resistance; Genetic and QTL mapping; Candidate genes. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00271/38244/36429.pdf |
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| Arzul, Isabelle; Langlade, Aime; Chollet, Bruno; Robert, Maeva; Ferrand, Sylvie; Omnes, Emmanuelle; Lerond, Sophie; Couraleau, Yann; Joly, Jean-pierre; Francois, Cyrille; Garcia, Celine. |
| Bonamia ostreae is an intracellular protistan parasite affecting flat oysters Ostrea edulis. It can be detected in juveniles but mortalities mainly affect oysters which are more than 2 years old. The parasite is usually observed inside haemocytes and sometimes free, notably in gill epithelia suggesting a parasite release through this organ. However, the infective form and ways of entry and release remain undetermined. Flat oysters incubate their larvae in their pallial cavity for 8–10 days before releasing them into the water column. Flat oysters in Bay of Quiberon in South Brittany (France) are known to be infected with B. ostreae since 1979 and is the most important area in France for O. edulis spat collection. Flat oysters incubating larvae were sampled... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Flat oyster; Ostrea edulis; Transmission; Larvae; Parasite life cycle. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00028/13898/12566.pdf |
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| Segretin, Angelique. |
| Les méthodes de classification sont largement utilisées en vision par ordinateur et reconnaissance des formes. La classification floue a d'ailleurs montré de bonnes performances dans ces domaines, non seulement dans la reconnaissance de classes en volume, mais aussi pour des formes fines, des droites, des courbes, et également des surfaces. L'idée est d'utiliser la classification automatique pour tenter de caractériser des types hémocytaires présents dans l'hémolymphe de l'huître plate. Actuellement, trois types d'hémocyte ont été définis, mais la difficulté d'établir un classement des cellules dans ces trois groupes, laisse penser qu'ils ne sont pas exhaustifs. Différentes approches de classification ont été tentées sur des mesures morphométriques de... |
| Tipo: Text |
Palavras-chave: Classification floue; Analyse d'image; Huître plate; Ostrea edulis; Hémocytes. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00033/14445/11744.pdf |
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| Morga, Benjamin; Renault, Tristan; Faury, Nicole; Chollet, Bruno; Arzul, Isabelle. |
| Bonamia ostreae is a protozoan, affiliated to the order Haplosporidia and to the phylum Cercozoa. This parasite is intracellular and infects haemocytes, cells notably involved in oyster defence mechanisms. Bonamiosis due to the parasite B. ostreae is a disease affecting the flat oyster, Ostrea edulis. The strategies used by protozoan parasites to circumvent host defence mechanisms remain largely unknown in marine bivalve molluscs. In the present work, in vitro experiments were carried out in order to study the interactions between haemocytes from O. edulis and purified parasite. B. ostreae. We monitored cellular and molecular responses of oyster haemocytes by light microscopy, flow cytometry and real-time PCR 1, 2, 4 and 8 h p.i. Light microscopy was used... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Haemocytes; Real-time PCR; Flow cytometry; Super oxide dismutase. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00037/14874/18001.pdf |
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| Lallias, Delphine; Gomez-raya, L.; Haley, C. S.; Arzul, Isabelle; Heurtebise, Serge; Beaumont, A. R.; Boudry, Pierre; Lapegue, Sylvie. |
| We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage... |
| Tipo: Text |
Palavras-chave: Statistical power; Survival analysis; QTL mapping; Disease resistance; Ostrea edulis. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6682.pdf |
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| Baud, Jean-pierre; Le Bayon, Nicolas; Haure, Joel; Nourry, Max; Palvadeau, Hubert; Penisson, Christian; Naciri-graven, Yamama; Goyard, Emmanuel; Barre, Marc. |
| As of 1985, Ifremer set up a selection programme for resistance to the protozoan Bonamia ostreae in European flat oysters (Ostrea edulis). Two selection lines, L85 and L89, from the third and second generation, respectively, were produced with 17 and 21 different families. The experimental design also included a mixed line (L85 x L89) of seven families and a control line of five families from Quiberon broodstock maintained without selection pressure. |
| Tipo: Text |
Palavras-chave: Croissance; Génétique; Huîtres plates; Ostrea edulis. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/1997/acte-3245.pdf |
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| Comesana, Pilar; Casas, Sandra M.; Cao, Asuncion; Abollo, Elvira; Arzul, Isabelle; Morga, Benjamin; Villalba, Antonio. |
| Farming of the flat oyster Ostrea edulis in Europe is severely constrained by the protozoan Bonamia ostreae. The introduction of the resistant species Crassostrea gigas has been a relief for the farmers, while the pilot programmes to select O. edulis strains resistant to bonamiosis performed in various countries can be seen as a promising strategy to minimise the effects of bonamiosis. However, the physiological bases of this differential susceptibility remain unknown. A search for an explanation of the intra and interspecific differences in oyster susceptibility to bonamiosis was accomplished by comparing some immune parameters among various O. edulis stocks and C gigas. On December 2003, naive and Bonamia-relatively resistant flat oysters from Ireland.... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Haemocyte; Phagocytosis; Respiratory burst; Nitric oxide. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00074/18501/16092.pdf |
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| Tige, Gilbert; Grizel, Henri; Langlade, Aime; Rabouin, Marie-agnès. |
| Les premiers stades connus du parasite ont été mis en évidence en Bretagne pendant les mois de juillet et d'août (GRIZEL et TIGE, 1977). Suite à ces observations, un essai a été réalisé en 1978 pour savoir si l'infestation avait bien lieu à ces dates ou si elle se faisait antérieurement sous une forme non décelée actuellement et subissant des transformations profondes dans les tissus de l'huître. Les résultats montrent que l'infestations se fait effectivement pendant l'été, à partir du mois de juillet jusqu'à la fin du mois d'août. Elle fut dans l'ensemble faible; les lots les plus infestés sont ceux qui ont séjourné le plus longtemps en zone contaminée. |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Marteilia refringens; Pathologie; Parasite. |
| Ano: 1979 |
URL: http://archimer.ifremer.fr/doc/1979/acte-6098.PDF |
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| Gerard, Andre; Naciri, Yamama; Coatanea, Denis; Martin, Anne-genevieve; Barret, Jean; Claude, Serge; Heurtebise, Serge; Devauchelle, Nicole; Joly, Jean-pierre; Goulletquer, Philippe; Baud, Jean-pierre; Nourry, Max; Bougrier, Serge; Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno; Le Deuff, Rose-marie; Peignon, Jean-marie. |
| La discussion générale organisée à la fin de la deuxième journée a fait ressortir les points suivants: 1- Les participants se sont déclarés satisfaits de la tenue de la réunion, tant dans son déroulement, que dans les échanges d'informations auxquels elle a donné lieu. La quantité et la qualité des résultats présentés a permis de mettre en évidence la puissance d'analyse que représente le travail en réseau. 2-L'importance de la valorisation des résultats a été soulignée à plusieurs reprises. La diffusion de l'information devrait se faire dans un premier temps par la voie de rapport internes à la DRV, puis par la voie d'articles publiés dans des revues scientifiques. Sur le programme "C. gigas triploïdes", une première diffusion des résultats obtenus en... |
| Tipo: Text |
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00049/15981/13423.pdf |
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| Goyard, Emmanuel; Lapegue, Sylvie; Cochennec, Nathalie; Baud, Jean-pierre; Langlade, Aime; Joly, Jean-pierre; Buestel, Dominique; Oheix, Jocelyne; Haure, Joel. |
| Le programme de sélection de l'huître plate a été initié en 1985 par des pathologistes de la Tremblade. La sélection ne portait que sur le seul critère de tolérance des huîtres plates au parasite Bonamia ostreae et correspondait plus à un outil pour étudier divers aspects de la parasitose qu'un un programme d'amélioration génétique visant la relance de la culture de l'huître plate. Dans le cadre du réseau REGEMO, ce programme a ensuite été repris par les généticiens de l'Ifremer : 1) rappel historique et définition d'un vocabulaire commun à l'ensemble des membres du réseau REGEMO. 2) Inventaire et bilan des opération menées ou en cours ( sur la dernière génération de sélection produite ne 1995, sur le matériel issu de cette génération et produit en 1997.... |
| Tipo: Text |
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00049/15977/13419.pdf |
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| Registros recuperados: 225 | |
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